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Image Search Results
Journal: Science translational medicine
Article Title: Epstein-Barr virus reprograms autoreactive B cells as antigen presenting cells in systemic lupus erythematosus
doi: 10.1126/scitranslmed.ady0210
Figure Lengend Snippet: ( A ) Representative examples of immunostaining patterns of SLE EBV + B cell-derived mAb on HEp-2 cells. Nuclear homogeneous includes both homogeneous and fine speckled staining patterns. ( B ) Representative examples of immunostaining of HC and MS EBV + B cell-derived mAbs on HEp-2 cells. ( C ) ELISA analysis of the reactivity of SLE, HC, and MS EBV + B cell-derived mAbs to prototypical SLE autoantigens, including the ANA screen ELISA (Nuclear Ag cocktail) and individual nuclear antigens, and for EBV EBNA1. As an assessment for potential polyreactive antibodies, LPS ELISAs were performed. The Nuclear Antigen (Ag) cocktail, comprising Sm (Smith), RNP/Sm, Scl-70, SS-A (Ro) (52 kDa and 60 kDa), SS-B (La), Jo-1, U1-SmRNP, CENP-B, dsDNA, Histones, and C1q was obtained from a commercial source different from that used for the individual antigens in the heatmap. N = number of mAbs tested per group.
Article Snippet: The nuclear antigen cocktail ELISA was performed according to the manufacturer’s instructions using the
Techniques: Immunostaining, Immunopeptidomics, Derivative Assay, Staining, Enzyme-linked Immunosorbent Assay